Principles and Process of Biotechnology - Genetic Engineering (Recombinant DNA technology) NEET Questions

Principles and Process of Biotechnology - Genetic Engineering (Recombinant DNA technology) MCQ Questions

13.
In pBR322 vector, ampR and tetR are:
A.
Selectable markers (antibiotic resistance genes)
B.
Origins of replication
C.
Recognition sites
D.
Promoter sequences
ANSWER :
A. Selectable markers (antibiotic resistance genes)
14.
Insertional inactivation of an antibiotic resistance gene helps in:
A.
Selecting recombinants from non-recombinants
B.
Replicating plasmid
C.
Cutting DNA
D.
Selecting transformants
ANSWER :
A. Selecting recombinants from non-recombinants
15.
Which of the following acts as a vector to deliver desired gene into plant cells?
A.
Escherichia coli
B.
Bacillus thuringiensis
C.
Salmonella typhimurium
D.
Agrobacterium tumefaciens
ANSWER :
D. Agrobacterium tumefaciens
16.
Retroviruses serve as ideal vectors for:
A.
Plant cell transformation
B.
Animal cell transformation / gene therapy
C.
Yeast transformation
D.
Bacterial cloning
ANSWER :
B. Animal cell transformation / gene therapy
17.
The Ti plasmid is found in:
A.
Bacillus thuringiensis
B.
Agrobacterium tumefaciens
C.
Escherichia coli
D.
Salmonella typhimurium
ANSWER :
B. Agrobacterium tumefaciens
18.
Selectable markers in cloning vectors are required because:
A.
They help identify and eliminate non-transformants while permitting growth of transformants
B.
They denature the DNA
C.
They synthesise DNA
D.
They cut the DNA
ANSWER :
A. They help identify and eliminate non-transformants while permitting growth of transformants