Principles and Process of Biotechnology - Genetic Engineering (Recombinant DNA technology) NEET Questions

Biology - Biotechnology and Its Applications

Principles and Process of Biotechnology - Genetic Engineering (Recombinant DNA technology) MCQ Questions


7.
DNA ligase is used in rDNA technology to:
A.
Synthesise DNA from RNA template
B.
Join DNA fragments by forming phosphodiester bonds
C.
Cut DNA at specific sites
D.
Denature DNA strands
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ANSWER :
B. Join DNA fragments by forming phosphodiester bonds
8.
Which enzyme is used to synthesise cDNA from mRNA?
A.
DNA polymerase III
B.
Restriction endonuclease
C.
Reverse transcriptase
D.
RNA polymerase
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ANSWER :
C. Reverse transcriptase
9.
EcoRI recognises and cuts the sequence:
A.
5'-AAGCTT-3'
B.
5'-CTGCAG-3'
C.
5'-GGATCC-3'
D.
5'-GAATTC-3'
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ANSWER :
D. 5'-GAATTC-3'
10.
The first artificial recombinant DNA molecule was constructed by:
A.
Cohen and Boyer
B.
Frederick Sanger
C.
Paul Berg (1972)
D.
Watson and Crick
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ANSWER :
C. Paul Berg (1972)
11.
In rDNA technology, plasmids serve as:
A.
Restriction enzymes
B.
Hosts
C.
Vectors
D.
Substrates for ligase
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ANSWER :
C. Vectors
12.
Which of the following is NOT a feature of a good cloning vector?
A.
Origin of replication (ori)
B.
Cloning sites (unique restriction sites)
C.
Multiple recognition sites for the same enzyme
D.
Selectable marker
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ANSWER :
C. Multiple recognition sites for the same enzyme