In Lassaigne's test, the sodium fusion extract is tested for the presence of nitrogen by treating it with freshly prepared ferrous sulphate solution, followed by:
A.
Addition of ammonium hydroxide alone
B.
Boiling with distilled water alone
C.
Acidification with dilute sulphuric acid, after boiling and cooling the solution
D.
Direct addition of concentrated nitric acid without any other reagent