Biotechnology and Its Applications NDA Questions

NDA BIOLOGY

Biotechnology and Its Applications MCQ Questions


1.
Which enzyme is used to cut DNA at specific sequences in recombinant DNA technology?
A.
Restriction endonuclease
B.
RNA polymerase
C.
DNA ligase
D.
DNA polymerase
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ANSWER :
A. Restriction endonuclease
2.
DNA ligase is used in recombinant DNA technology to:
A.
Cut DNA at specific sites
B.
Amplify DNA in vitro
C.
Join two DNA fragments
D.
Separate DNA by size
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ANSWER :
C. Join two DNA fragments
3.
Which of the following is the most widely used cloning vector in bacteria?
A.
pBR322 plasmid
B.
Ti plasmid
C.
Cosmid
D.
Lambda phage
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ANSWER :
A. pBR322 plasmid
4.
The process of introducing recombinant DNA into a bacterial host cell is called:
A.
Transduction
B.
Transformation
C.
Transfection
D.
Conjugation
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ANSWER :
B. Transformation
5.
Polymerase Chain Reaction (PCR) amplifies DNA using:
A.
Restriction endonuclease
B.
RNA polymerase
C.
DNA ligase
D.
Taq DNA polymerase
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ANSWER :
D. Taq DNA polymerase
6.
The three steps in each PCR cycle are:
A.
Denaturation, Annealing, Extension
B.
Replication, Transcription, Translation
C.
Isolation, Digestion, Ligation
D.
Ligation, Transformation, Selection
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ANSWER :
A. Denaturation, Annealing, Extension