Biotechnology and Its Applications NDA Questions

NDA BIOLOGY

Biotechnology and Its Applications MCQ Questions


7.
A palindromic sequence in DNA means:
A.
The sequence codes for a palindrome protein
B.
The DNA does not have restriction sites
C.
Both strands read the same sequence in 5'→3' direction
D.
Only one strand is cut
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ANSWER :
C. Both strands read the same sequence in 5'→3' direction
8.
Gel electrophoresis separates DNA fragments on the basis of:
A.
Colour and density
B.
Protein content
C.
Temperature and pressure
D.
Size and charge
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ANSWER :
D. Size and charge
9.
Which organism is commonly used as a host for recombinant protein production?
A.
Streptococcus pneumoniae
B.
Plasmodium falciparum
C.
Escherichia coli
D.
Mycobacterium tuberculosis
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ANSWER :
C. Escherichia coli
10.
The first recombinant DNA molecule was created by:
A.
Frederick Sanger
B.
Watson and Crick
C.
Stanley Cohen and Herbert Boyer
D.
Kary Mullis
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ANSWER :
C. Stanley Cohen and Herbert Boyer
11.
Humulin, the genetically engineered human insulin, was first produced by:
A.
Novo Nordisk (using yeast)
B.
Pfizer (using CHO cells)
C.
GlaxoSmithKline (using Bacillus)
D.
Eli Lilly (using E. coli)
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ANSWER :
D. Eli Lilly (using E. coli)
12.
In production of recombinant human insulin, the two polypeptide chains A and B are:
A.
Extracted directly from human pancreas
B.
Produced together as a single chain
C.
Synthesised chemically without bacteria
D.
Produced separately in E. coli and combined by disulfide bonds
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ANSWER :
D. Produced separately in E. coli and combined by disulfide bonds