Biotechnology UPSC Questions

Biotechnology MCQ Questions

7.
What is the name of the enzyme that forms hydrogen bonds between sticky ends of DNA?
A.
DNA polymerase
B.
Restriction endonuclease
C.
DNA ligase
D.
Chitinase
ANSWER :
C. DNA ligase
8.
Which vector is commonly used for cloning genes into plants?
A.
Retrovirus
B.
pBR322
C.
Ti plasmid
D.
Plasmid DNA
ANSWER :
C. Ti plasmid
9.
Which technique is used to amplify DNA in vitro?
A.
Electrophoresis
B.
PCR
C.
Micro-injection
D.
Biolistics
ANSWER :
B. PCR
10.
In the context of the developments in Bioinformatics, the term โ€˜transcriptomeโ€™, sometimes seen in the news, refers to

UPSC - 2016

A.
a range of enzymes used in genome editing
B.
the full range of mRNA molecules expressed by an organism
C.
the description of the mechanism of gene expression
D.
a mechanism of genetic mutation taking place in cells
ANSWER :
B. the full range of mRNA molecules expressed by an organism
11.
Why is calcium treatment used in the transformation of bacterial cells?
A.
To break DNA
B.
To open plasmids
C.
To increase membrane permeability
D.
To inactivate endonucleases
ANSWER :
C. To increase membrane permeability
12.
What is downstream processing in biotechnology?
A.
Transfer of DNA into host cells
B.
Separation and purification of the product
C.
Replication of DNA
D.
Insertion of genes
ANSWER :
B. Separation and purification of the product