Biotechnology UPSC Questions

Biology

Biotechnology MCQ Questions


7.
What is the name of the enzyme that forms hydrogen bonds between sticky ends of DNA?
A.
DNA polymerase
B.
Restriction endonuclease
C.
DNA ligase
D.
Chitinase
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ANSWER :
C. DNA ligase
8.
Which vector is commonly used for cloning genes into plants?
A.
Retrovirus
B.
pBR322
C.
Ti plasmid
D.
Plasmid DNA
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ANSWER :
C. Ti plasmid
9.
Which technique is used to amplify DNA in vitro?
A.
Electrophoresis
B.
PCR
C.
Micro-injection
D.
Biolistics
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ANSWER :
B. PCR
10.
In the context of the developments in Bioinformatics, the term ‘transcriptome’, sometimes seen in the news, refers to

UPSC - 2016

A.
a range of enzymes used in genome editing
B.
the full range of mRNA molecules expressed by an organism
C.
the description of the mechanism of gene expression
D.
a mechanism of genetic mutation taking place in cells
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ANSWER :
B. the full range of mRNA molecules expressed by an organism
11.
Why is calcium treatment used in the transformation of bacterial cells?
A.
To break DNA
B.
To open plasmids
C.
To increase membrane permeability
D.
To inactivate endonucleases
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ANSWER :
C. To increase membrane permeability
12.
What is downstream processing in biotechnology?
A.
Transfer of DNA into host cells
B.
Separation and purification of the product
C.
Replication of DNA
D.
Insertion of genes
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ANSWER :
B. Separation and purification of the product