Biotechnology UPSC Questions

Biology

Biotechnology MCQ Questions


13.
What is the function of the origin of replication in a cloning vector?
A.
It provides antibiotic resistance
B.
It initiates replication of DNA
C.
It facilitates ligation
D.
It expresses foreign genes
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ANSWER :
B. It initiates replication of DNA
14.
Which gene is inactivated in insertional inactivation to identify recombinants?
A.
Tetracycline resistance gene
B.
Ampicillin resistance gene
C.
β-galactosidase
D.
Chloramphenicol resistance gene
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ANSWER :
C. β-galactosidase
15.
What type of sequences do restriction enzymes recognize to cut DNA?
A.
Random sequences
B.
Start codons
C.
Palindromic sequences
D.
Stop codons
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ANSWER :
C. Palindromic sequences
16.
Which method is used to directly introduce DNA into the nucleus of animal cells?
A.
Biolistics
B.
Electroporation
C.
Heat shock
D.
Micro-injection
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ANSWER :
D. Micro-injection
17.
Which method uses high velocity particles to introduce DNA into plant cells?
A.
Micro-injection
B.
Gene gun
C.
Electroporation
D.
Transformation
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ANSWER :
B. Gene gun
18.
Which enzyme is used to remove RNA during DNA isolation?
A.
Protease
B.
Lysozyme
C.
Ribonuclease
D.
Chitinase
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ANSWER :
C. Ribonuclease