Biotechnology UPSC Questions

Biology

Biotechnology MCQ Questions


13.
Why is calcium treatment used in the transformation of bacterial cells?
A.
To break DNA
B.
To open plasmids
C.
To increase membrane permeability
D.
To inactivate endonucleases
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ANSWER :
C. To increase membrane permeability
14.
What is downstream processing in biotechnology?
A.
Transfer of DNA into host cells
B.
Separation and purification of the product
C.
Replication of DNA
D.
Insertion of genes
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ANSWER :
B. Separation and purification of the product
15.
What is the function of the origin of replication in a cloning vector?
A.
It provides antibiotic resistance
B.
It initiates replication of DNA
C.
It facilitates ligation
D.
It expresses foreign genes
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ANSWER :
B. It initiates replication of DNA
16.
Consider the following statements:
1. Genetic changes can be introduced in the cells that produce eggs or sperms of a prospective parent.
2. A person's genome can be edited before birth at the early embryonic stage.
3. Human induced pluripotent stem cells can be injected into the embryo of a pig.
Which of the statements given above is/are correct?
UPSC - 2020
A.
1 only
B.
2 and 3 only
C.
2 only
D.
1, 2 and 3
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ANSWER :
D. 1, 2 and 3
17.
Which gene is inactivated in insertional inactivation to identify recombinants?
A.
Tetracycline resistance gene
B.
Ampicillin resistance gene
C.
β-galactosidase
D.
Chloramphenicol resistance gene
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ANSWER :
C. β-galactosidase
18.
What type of sequences do restriction enzymes recognize to cut DNA?
A.
Random sequences
B.
Start codons
C.
Palindromic sequences
D.
Stop codons
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ANSWER :
C. Palindromic sequences